M-MLV Reverse Transcriptase
MMLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (MMLV RT) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV RT will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase. The difference between this to the general M-MLV RT is that the capacity to endure the heat is enhanced. It can remain the 100% activity at 50°C, it can also keep more than 80% activity even at 55°C.
M-MLV Reverse Transcriptase
lack RNase H activity: Weak RNase H activity High cDNA yield, can get more full length cDNA.
thermal stable: the optimum reaction temperature is 50°C, the highest is 60°C. Can overcome the template RNA secondary structure, and finish the reverse transcriptase experiment smoothly.
wide temperature range: can reverse transcript from 37-60C, with more than 80% of the highest activity at 42°C-55°C customer can choose the reaction temperature freely.
Strong amplification activity: Gene mutation enhanced the binding capacity of the enzyme and RNA. Increased the amplification speed, can obtain the quality cDNA, suitable for cDNA library construction.
M-MLV Reverse Transcriptase, RNase H Minus 200U/μl, supplied with 5x Reaction Buffer
M-MLV Reverse Transcriptase, wide type, RNase H postive. 200U/μl, supplied with 5x Reaction Buffer. High activity.
Thermal Stable reverse transcriptase, RNase H Minus, reaction temperature up to 55°C, 200U/μl
Thermal super stable reverse transcriptase, RNase H Minus, reaction temperature up to 60°C, 200U/μl
HiFi II M-MLV reverse transcriptase, RNase H Minus, for IVD use
HiFi II M-MLV reverse transcriptase, RNase H Minus, for IVD use, Lyophilized Powder
HiFi III M-MLV Reverse Transcriptase(RNase H-) customized M-MLV for COVID
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